Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis
Figure 3
(a) Cucumber mixoploid (2x/4x) seedling after in vitro polyploidization by oryzalin. (b) C. sativus (2x) × C. melo microcallus after in vivo pollination. (c) C. sativus (2x/4x) × C. melo plant after in vivo pollination. (d) C. sativus (2x) × C. melo microcallus after in vitro pollination. (e) C. sativus (2x/4x) × C. melo microcallus after in vitro pollination. (f) Differentiation of cucumber mixoploid protoplasts. (g) Microcallus of cucumber mixoploid protoplasts. (h) The viability of protoplasts isolated from cucumber mixoploid mesophyll after FDA staining. Bars in (b, c, e, g): 500 μm and in (g, f): 100 μm.
