Cytokines and Growth Factors Stimulate Hyaluronan Production: Role of Hyaluronan in Epithelial to Mesenchymal-Like Transition in Non-Small Cell Lung Cancer
Figure 3
Enhanced HA production promotes mesenchymal-like phenotype in H358 cells. H358 cells were stably transfected with either vector only acting as a control or HAS3 gene. Stable transfectants were assessed for (a) HAS3 mRNA expression by quantitative real time RT-PCR; (b) total HA production; (c) HA-dependent matrix visualized by particle exclusion assay; (d) expression of E-cadherin ((b) and (e)) and vimentin ((c) and (f)) by immunocytochemistry using the antibody against E-cadherin and vimentin and counterstained using ,6-diamidino-2-phenylindole. Cells in (a) and (d) were taken using a phase-contrast microscope. Scale bar: 10 m. Three experiments analyzed in triplicate were carried out for (a) and (b). The data shown is a representative. (Values are , , .)
