BioMed Research International

Research Article

The Protection of Hepatocyte Cells from the Effects of Oxidative Stress by Treatment with Vitamin E in Conjunction with DTT

Table 1

Effect of vitamin E and DTT on total GSH content, LDH leakage, and TBARS production in rat hepatocytes with TBH treatment.
TreatmentTotal GSH (nmol/mg protein)LDH leakage (%)TBARS (nmol/mg protein)
Untreated 4 7 . 7 ± 4 . 5 a 1 . 2 ± 0 . 6 a 0 . 6 6 ± 0 . 0 9 a b
TBH (1.0 mM) 1 9 . 5 ± 8 . 5 b c 4 3 . 8 ± 7 . 6 b 1 . 3 1 ± 0 . 4 1 a c
TBH (2.0 mM) 4 . 1 ± 1 . 2 d 7 6 . 2 ± 1 3 . 8 c 3 . 9 0 ± 0 . 3 1 d
Vitamin E (100  𝜇 M) + TBH (2.0 mM) 9 . 1 ± 0 . 2 b 7 . 6 ± 2 . 2 a 0 . 4 1 ± 0 . 1 1 b
EGTA (15 mM) + TBH (2.0 mM) 2 . 1 ± 0 . 1 d 6 2 . 8 ± 2 . 2 c 2 . 7 3 ± 0 . 5 1 e
DTT (5 mM) + TBH (2.0 mM) 2 9 . 7 ± 3 . 5 c e 2 6 . 6 ± 1 . 7 e 1 . 7 5 ± 0 . 2 0 c
Vitamin E (100  𝜇 M) + DTT (5 mM) + TBH (2.0 mM) 3 6 . 9 ± 4 . 2 a e 2 . 8 ± 1 . 1 a 0 . 5 5 ± 0 . 0 6 b
Values are expressed as means ± SD ( 𝑛 = 3 - 4 ). Means in the same column not sharing the same superscripts differ significantly ( 𝑃 < . 0 5 ).