Research Article

Microarray Detection Call Methodology as a Means to Identify and Compare Transcripts Expressed within Syncytial Cells from Soybean (Glycine max) Roots Undergoing Resistant and Susceptible Reactions to the Soybean Cyst Nematode (Heterodera glycines)

Table 4

An analysis compared the 3 dpi syncytia undergoing an incompatible reaction directly to the 3 dpi compatible syncytium samples. Selected genes that were unique to the 3 dpi syncytia undergoing an incompatible reaction but that were not differentially expressed as compared directly to the 3 dpi compatible syncytium samples (Figure 8(h)) comprising the Disease and Defense, Signaling, and Transcription categories are provided.

I-3 dpi genes in the comparison of I-3 dpi to C-3 dpi

Probe set IDPublic IDAvg 𝑃 -valueGene

Disease & defense

Gma.4886.2.S1_atAW2346240.005201937haem peroxidase
Gma.405.1.A1_atAI4434110.008396837leucine-rich repeat family protein (DRT100)
Gma.2044.2.S1_atBE8212300.011411572abscisic stress ripening-like protein
GmaAffx.92230.1.A1_s_atCF8079550.012305657thaumatin-like protein PR-5b
Gma.7542.2.S1_atCA9367640.016772715defender against cell death 1 (DAD-1)
Gma.8449.1.S1_s_atAF002258.10.019563038CoA ligase 4
GmaAffx.14986.1.S1_atBE6578890.020669698PHOSPHATE-INDUCED 1 (phi-1)
GmaAffx.2203.1.S1_atCD4157450.020669698cadmium-induced protein
GmaAffx.91141.1.S1_atCF8068660.020828644peroxidase 1 precursor
GmaAffx.46214.1.S1_atBE6592660.022934167polyphenol oxidase

Signal transduction

Gma.13604.1.S1_atCD4015370.011411572protein kinase
GmaAffx.56323.1.S1_atBU7642140.01212639protein kinase
Gma.6092.1.S1_atBI9687570.012305657COP9 signalosome subunit 3
Gma.5162.1.A1_atBI9711560.013412317Curculin-like (mannose-binding) lectin protein kinase
Gma.4455.3.S1_atCB0636320.014076915PROTEIN KINASE 2B (APK2B)
GmaAffx.66511.1.S1_atAW3509170.019563038calcium and calmodulin-dependent protein kinase (ATCDPK1)
GmaAffx.34312.1.S1_atAI9657350.023684433protein phosphatase 2C (PP2C)
GmaAffx.64402.1.S1_atAW3172820.025399823leucine-rich repeat
Gma.4801.1.S1_s_atBU5486080.028086024protein phosphatase 1 (PP1)
Gma.11291.1.S1_atAW3512070.028086024inositol 1,3,4-trisphosphate 5/6-kinase
GmaAffx.73451.1.S1_atBG0468890.034312943CALMODULIN-RELATED PROTEIN 2

Transcription

GmaAffx.89077.1.A1_s_atCK6056470.009323331CONSTANS interacting protein 2a
Gma.3996.1.S1_atAW3949460.014307059WRKY52
Gma.9678.1.S1_atCD4048940.016707249RNA polymerase II
Gma.12330.2.S1_s_atBI9727580.027055562pathogenesis-related transcriptional factor
Gma.6838.1.S1_atAW3496330.027055562NIM1-like protein 1 (NPR-1)
Gma.16645.1.S1_atBM1434290.028086024Arabidopsis NAC domain-containing protein 1 (ATAF1)