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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 525034, 12 pages
http://dx.doi.org/10.1155/2010/525034
Research Article

The Vacuolar-Type -ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals

1Research Unit Nutritional Physiology “Oskar Kellner”, Research Institute for the Biology of Farm Animals (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany
2Research Institute of Animal Production, Slovak Agricultural Research Centre, Nitra, Komenskeho 73, 041 81 Kosice, Slovakia
3Institute of Farm Animal Sciences and Technology, University of Rostock, Justus-Von Liebig-Weg 8, 18059 Rostock, Germany

Received 1 July 2009; Accepted 13 October 2009

Academic Editor: Kenichiro Kitamura

Copyright © 2010 Judith Kuzinski et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In this study, the effect of metabolic inhibition (MI) by glucose substitution with 2-deoxyglucose (2-DOG) and/or application of antimycin A on ovine rumen epithelial cells (REC) vacuolar-type -ATPase (v -ATPase) activity was investigated. Using fluorescent spectroscopy, basal of REC was measured to be in -free, glucose-containing NaCl medium. MI induced a strong reduction ( pH units) with a more pronounced effect of 2-DOG compared to antimycin A ( versus pH units). Treatment with foliomycin, a specific v -ATPase inhibitor, decreased REC by pH units. After MI induction, this effect was nearly abolished ( pH units). In addition, membrane-associated localization of v -ATPase B subunit disappeared. Metabolic control of v -ATPase involving regulation of its assembly state by elements of the glycolytic pathway could provide a means to adapt REC ATP consumption according to energy availability.