The Vacuolar-Type -ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals
Effect of the -ATPase inhibitor foliomycin before and after metabolic inhibition (MI) induced by glucose replacement with 2-DOG and application of antimycin A on p of ruminal epithelial cells (REC). Foliomycin was added to REC suspended in either HEPES-buffered -free Na-medium containing 5 mM glucose (control; = 6) or in glucose-free media with 2-DOG and antimycin A ( = 7). A: The figure shows original traces from one experiment. B: To summarize the foliomycin effects, the mean p reduction from the p measured in respective control medium without inhibitor has been calculated and is shown for both conditions. Values are means SE; versus control.