BioMed Research International / 2010 / Article / Fig 7

Research Article

The Vacuolar-Type -ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals

Figure 7

Immunoblot of the v H + -ATPase subunit B ( 60 kDa) and the N a + / K + -ATPase 𝛼 subunit ( 110 kDa) in ovine REC. Protein extracts were prepared from REC isolated during series 1 (lane 1 and 4) or series 2 (lane 2, 3, 5 and 6) of the experiments. For the N a + / K + -ATPase 𝛼 subunit, a band at the expected size of approximately 110 kDa was always detected (lane 4–6). In contrast, a clear 60 kDa-band for H + -ATPase subunit B protein was found with protein extracts from series 1 only (lane 1). With protein extracts from REC with low metabolic activity, staining was very weak (lane 2) or completely absent (lane 3).

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