Figure 1: HIV-based transfer vectors plasmid used in this study. The internal CMV promoter drives the expression of the codon-optimized HI V J R - F L gp120 Envelope protein and the murine GM-CSF (mGM-CSF). The packaging signal ( Ψ ) , primer binding site (PBS), major splice donor (SD) and acceptor (SA) sites, bovine Growth Hormone polyadenylation signal (bHGpA), central polypurine tract (cPPT), internal ribosomal entry site from poliovirus (pIRES), and Woodchuck posttranscriptional regulatory element (WPRE) are indicated.