Research Article

The Adenovirus Type 3 Dodecahedron's RGD Loop Comprises an HSPG Binding Site That Influences Integrin Binding

Figure 3

FACS analysis of Bs-Dd and mutant binding at 4 C to HSPG expressing HeLa cells and HSPG deficient CHO-2241. (a) Particle binding to HeLa cells. Dodecahedra (wt or mutants) were incubated with HeLa cells in PBS 1 mM CaC l 2 preincubated or not with S36578 drug (upper right and left panels, resp.). Lower panels show individual responses of the different mutants with or without S36578. (b) Particle binding to CHO-2241 cells under the same conditions used above. Dodecahedra were allowed to bind to CHO-2241 cells preincubated or not with S36578 drug (right and left panels, resp.). Bound particles were detected by an antibody serum directed against the Ad3 penton base.