TY - JOUR
A2 - Dovc, Peter
AU - Dall'Olio, Stefania
AU - Fontanesi, Luca
AU - Nanni Costa, Leonardo
AU - Tassinari, Marco
AU - Minieri, Laura
AU - Falaschini, Adalberto
PY - 2010
DA - 2010/07/14
TI - Analysis of Horse Myostatin Gene and Identification of Single Nucleotide Polymorphisms in Breeds of Different Morphological Types
SP - 542945
VL - 2010
AB - Myostatin (MSTN) is a negative modulator of muscle mass. We characterized the horse (Equus caballus) MSTN gene and identified and analysed single nucleotide polymorphisms (SNPs) in breeds of different morphological types. Sequencing of coding, untranslated, intronic, and regulatory regions of MSTN gene in 12 horses from 10 breeds revealed seven SNPs: two in the promoter, four in intron 1, and one in intron 2. The SNPs of the promoter (GQ183900:g.26T>C and GQ183900:g.156T>C, the latter located within a conserved TATA-box like motif) were screened in 396 horses from 16 breeds. The g.26C and the g.156C alleles presented higher frequency in heavy (brachymorphic type) than in light breeds (dolichomorphic type such as Italian Trotter breed). The significant difference of allele frequencies for the SNPs at the promoter and analysis of molecular variance (AMOVA) on haplotypes indicates that these polymorphisms could be associated with variability of morphology traits in horse breeds.
SN - 2314-6133
UR - https://doi.org/10.1155/2010/542945
DO - 10.1155/2010/542945
JF - Journal of Biomedicine and Biotechnology
PB - Hindawi Publishing Corporation
KW -
ER -