A Styrene-alt-Maleic Acid Copolymer Is an Effective Inhibitor of R5 and X4 Human Immunodeficiency Virus Type 1 Infection
Figure 3
alt-PSMA inhibits infection of P4-R5 MAGI cells by HIV-1-infected cells. (a) HIV-1 strain IIIB-infected Sup-T1 T cells were incubated for 10 min in the absence or presence of alt-PSMA or DS prior to 1 : 10 dilution and incubation with P4-R5 MAGI cells for 2 h (as described in Section 2). (b) Mitomycin C-treated HIV-1 strain IIIB-infected Sup-T1 T cells were incubated for 2 min in the absence or presence of alt-PSMA or DS prior to 1 : 10 dilution and incubation with P4-R5 MAGI cells for 2 h (as described in Section 2). Infectivity remaining was expressed relative to mock-treated, HIV-1-infected cells and graphed against the compound concentration achieved during the 2-h incubation with P4-R5 MAGI cells. Each concentration was examined in triplicate in two independent assays.
