A Styrene-alt-Maleic Acid Copolymer Is an Effective Inhibitor of R5 and X4 Human Immunodeficiency Virus Type 1 Infection
Figure 6
alt-PSMA washout does not increase HIV-1 BaL or IIIB infection. P4-R5 MAGI cells were incubated for 1 h with predetermined IC90 concentrations of alt-PSMA (0.03 mg/mL, BaL; 0.02 mg/mL, IIIB), PSS (0.1 mg/mL, BaL; 2.29 mg/mL, IIIB), or DS (0.031 mg/mL, BaL; 0.0028 mg/mL, IIIB). Following exposure, cells were washed thoroughly, provided with new media for 2 h or 4 h, and subsequently infected with HIV-1 strains BaL (a) or IIIB (b) for 1 h. As a control, compound, virus, and cells were simultaneously incubated for 1 h (labeled “S” on each graph). Levels of infection are expressed relative to mock-treated, HIV-1 infected cells. Each concentration was examined in triplicate in two independent assays. The asterisk (*) indicates a significant difference compared to infected, mock-exposed control cells ().
