BioMed Research International / 2010 / Article / Fig 2

Research Article

Increasing a Robust Antigen-Specific Cytotoxic T Lymphocyte Response by FMDV DNA Vaccination with IL-9 Expressing Construct

Figure 2

Effect of IL-9 as adjuvant on humoral and cell proliferative responses. (a) Serum samples from six mice per immunized group were analyzed for specific antibodies against VP1 by ELISA. Mouse IgG with a known concentration was serially diluted and a standard curve was obtained for concentration of specific binding. (b) T cells were isolated from mice (6 per group) of all groups on day 7 after the third immunization and stimulated with VP1 recombinant protein. A cell proliferation response was analyzed using MTT and expressed as stimulation index. Data showed are representatives from three independent experiments. 𝑃 < . 0 5 compared with pcD-VP1 group.

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