BioMed Research International / 2010 / Article / Fig 4

Research Article

Increasing a Robust Antigen-Specific Cytotoxic T Lymphocyte Response by FMDV DNA Vaccination with IL-9 Expressing Construct

Figure 4

Analysis of antigen-specific cytokine productions in CD8+ T cells by FACS. (a) CD8+ T cells isolated from the spleen of C57BL/6 mice on day 7 after the final boost were stimulated with VP1 peptide for 4 h in culture. Intracellular staining for IFN- 𝛾 , perforin, and IL-17 in CD8+ T cells was performed. (b) The summaries of percentage were shown in the means of the three independent experiments.

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