Two-Dimensional Liquid Chromatography Technique Coupled with Mass Spectrometry Analysis to Compare the Proteomic Response to Cadmium Stress in Plants

Table 1

Proteins regulated by Cd treatment in leaves of Populus nigra clone “Poli”, separated by 2D-LC.

Nos.^𝑎	Homologous protein^𝑏	Accession number^𝑏	Measured pI interval^𝑐	Predicted pI/Mr (Da)^𝑐	Score / coverage (%)^𝑑	Ratio^𝑒	ESTs poplar^𝑓
LC1	Aquaporin	NIP12_ARATH	<4.00	8.63/31249	56/40	+5.6	P047H06
LC2	Dehydration-responsive protein	gi|18405331	4.03–4.33	8.60/67472	60/35	+3.4	P082D06
LC3	Predicted protein	gi|162695261	5.11–5.41	6.20/45886	69/32	−7.2	T020D11
LC4	Acetyl coenzime A carboxylase	gi|74272305	5.41–5.71	5.60/120177	69/35	−3.5	Q001C04
LC5	NADPH isocitrate dehydrogenase	gi|169989	5.71–5.99	6.13/49508	51/28	+4.4	N006H08
LC6	Thylakoidal processing peptidase 2	TPP2_ARATH	6.29–6.59	6.54/40581	56/21	+4.8	T040C06
LC7	Hypothetical protein	gi|125603717	6.29–6.59	6.67/47148	71/26	−3.0	Y004E07
LC8	Predicted protein	gi|168009327	7.49–7.79	7.22/42542	69/40	+5.3	V017A06
LC9	Chaperone ClpB	CLPB_RHOBA	>8.00	5.06/97498	110/56	+8.4	P058E06
LC10	Rubisco large subunit	gi|33636375	>8.00	5.13/6207	69/80	−8.5	No match
LC11	Aldo/keto reductase protein	gi|15232354	>8.00	6.46/35185	64/35	+4.5	V056F06
LC12	Cytochrome P450	C71BT_ARATH	>8.00	8.06/56318	105/64	+7.3	M122H12
LC13	ATP-dependent Clp protein	CLPR1_ARATH	>8.00	8.81/42942	51/35	+4.7	UM61TE04
LC14	Cysteine proteinase inhibitor 2	CYT2_ORYSJ	>8.00	8.82/16550	56/25	+3.2	FL_GENBANK_63
LC15	Hydroxyacylglutathione hydrolase 1	GLO2M_ARATH	>8.00	8.83/36933	51/40	−4.6	S065B02
LC16	Hypothetical protein	gi|38175624	>8.00	9.10/27188	69/36	−4.5	N054D02
LC17	GTP binding protein	gi|145359760	>8.00	9.46/20079	60/40	−3.2	K027P68
LC18	Protein kinase APK1B	APK1B_ARATH	>8.00	9.37/45946	56/37	+5.3	N041C12
LC19	CRS2A chloroplastic intron splicing facilitator	CRS2A_ARATH	>8.00	9.40/27356	69/60	+3.5	K028P24
LC20	Nucleotide translocator	gi|444790	>8.00	9.78/43995	92/43	−7.4	T027D03

^aThe nos. refer to the spot numbers as given in Figure 3. ^bPutative protein identification and accession number for the closest match in the SWISS PROT and NCBI databases. ^cpI values as estimated from the 2D-LC together with the predicted pI and Mr of the closest match in the databases. ^dScore and percentage of coverage of the matching peptide sequence tags. ^eRatio between values of the peak areas of the proteins from treated leaves (50 𝜇M CdSO₄) and untreated leaves (0 𝜇M CdSO₄) obtained from the Deltavue software. ^fMatches with the poplar EST database.