Two-Dimensional Liquid Chromatography Technique Coupled with Mass Spectrometry Analysis to Compare the Proteomic Response to Cadmium Stress in Plants

Table 2

Functional classes and putative locations of the proteins identified by MS.

Protein class	Protein name	Localization	Predicted function or domains
Uptake	Aquaporin NIP-2	Plasma membrane	Channel/ pore transporter
Proteolysis	Thylakoidal processing peptidase	Chloroplast membrane	Peptidase activity
Stress proteins	Cysteine proteinase inhibitor	Cytosol	Inhibition of exogenous proteases such as those present in digestive tracks of insects and nematodes
	Aldo/keto reductase	Cytosol	Oxidoreductase activity involved in response to cadmium ions
	Cytochrome P450	Endoplasmic reticulum	Ferulate 5-hydrolase activity, monoxygenase activity
	Dehydration responsive protein	Golgi apparatus	Biological process unknown
	ClpB, chloroplastic	Chloroplast	ATP binding, ATPase activity
	ATP-dependent Clp	Chloroplast	Protease activity
Metabolism	NADPH isocitrate dehydrogenase	Mitochondrion	TCA cycle
	CRS2A, chloroplastic intron splicing facilitator	Chloroplast	Aminoacyl-tRNA hydrolase activity
	Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit	Chloroplast	Calvin cycle
	Nucleotide translocator	Mitochondrial inner membrane	ATP  :  ADP antiporter activity
	Acetyl-CoA carboxylase	Mitochondrion	Fatty acid metabolism
	Predicted protein	Mitochondrion	Succinyl-CoA synthetase subunit beta
Regulatory protein	APK1B kinase	Chloroplast	Serine/ threonine activity
	GTP binding protein	Mitochondrion	Protein targeting, sorting and translocation
	Predicted protein	—	Serine/ threonine kinase domain
Glutathione pathway	Hydroxyacylglutathione hydrolase 1	Mitochondrion	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl glutathione to form glutathione and D-lactic acid

Predictions were obtained by checking putative protein acquisition with the annotation in SWISS-PROT, NCBI, and databases.