Research Article

Increased Expression of Ganglioside GM1 in Peripheral CD4+ T Cells Correlates Soluble Form of CD30 in Systemic Lupus Erythematosus Patients

Figure 1

Time kinetics of GM1 expressions on PHA-activated T cells. Peripheral mononuclear cells were stimulated with 1  𝜇 g/ml of PHA and maintained with IL-2-containing medium for two weeks. GM1 expression on memory T cells were detected with FITC-CTB and PE-CD45RO antibody on days 0, 2, 5, 8, 11 and 14 after stimulation (a). The mean fluorescence intensity (MFI) of GM1 were measured on CD45RO+/CD4+ memory T cells (b) and CD4 + T cells (c). Each experiment was done in triplicate. Error bars represent S.E.M.
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(a)
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(b)
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(c)