Intranasal Immunization with Chitosan/pCAGGS-flaA Nanoparticles Inhibits Campylobacter jejuni in a White Leghorn Model
Figure 3
In vitro gene expression of chitosan-encapsulated pCAGGS-flaA in COS-7 cell line by immunofluorescent assay. Ability of constructed chitosan-DNA vaccine complex to express flaA in eukaryotic cells (COS-7 cell line) transfected with pCAGGS-flaA (a), and the negative control pCAGGS (b) was evaluated. After 48 h transfection, only pCAGGS-flaA-transfected cells expressed protein flaA, which was detected by fluorescent microscopy.
