Mitoxantrone Loaded Superparamagnetic Nanoparticles for Drug Targeting: A Versatile and Sensitive Method for Quantification of Drug Enrichment in Rabbit Tissues Using HPLC-UV
Table 4
Recovery of MTO after extraction of different matrices using different cartridges. Results vary extraordinarily, FF-bound MTO in liver tissue requires special solid phase.
Matrix
Cartridge
Cartridge treatment after extraction(1)
Recovery [%]
FF-MTO
Chromabond HR-X (Machery&Nagel)
Water, MeOH
21,2%
FF-MTO
Chromabond HR-X (Machery&Nagel)
1N HCl, MeOH
22%
FF-MTO
Oasis MCX (Waters)
HCOOH (2%), MeOH, Elution with 5% NH3
0,9%
FF-MTO
LiChrolut En (Merck)
Water, MeOH
FF-MTO
1: Bond Elut PPL (Varian)
MeOH, HCOOH (2%).
1:75%
Rinse with HCOOH (2%).
2: Bond Elut Plexa (Varian)
Elution with (2%) HCOOH in
2:81%
MeOH.
FF-MTO + 0.5 g porcine Liver
Sep-Pak-Plus C18 (Waters)
MeOH, 1N HCl
FF-MTO + 0.5 g porcine Liver
LiChrolut En (Merck)
Water, MeOH
n.d.(2)
FF-MTO + 0.5 g porcine Liver
1: Bond Elut PPL (Varian)
MeOH, HCOOH (2%)..
1:31%
Rinse with HCOOH (2%).
2: Bond Elut Plexa (Varian)
Elution with (2%) HCOOH in
2:86%
MeOH
(1)Three times extracting with: 500 L water, 200 L MeOH, 50 L ascorbic acid (20%) in citrate buffer (pH 3.0), 200 L formic acid, 100 L 20% trichloroacetic acid, 400 L chloroform in an ultrasonic bath. (2)n.d.: not detectable. MTO could not be fixated on the cartridge.
