Table 1: Media and incubation conditions used for enumeration of representative microbes in the human large intestine.

Microbial groupMediumIncubation conditions and timeColonies formed

Total aerobesBrain heart infusion agarAerobic, 37C, 24 hWhite
Total anaerobesBrain heart infusion agarAnaerobic, 37C, 72 hWhite
Escherichia coliMc Conkey agarAerobic, 43C, 24 hRed-purple
Staphylococcus sp.Mannitol Salt agarAerobic, 37C, 48 hWhite with yellow/purple zone
Lactobacillus sp.Rogosa agarAnaerobic, 37C, 72 hWhite