Research Article

Dynamic Metabolic Flux Analysis Demonstrated on Cultures Where the Limiting Substrate Is Changed from Carbon to Nitrogen and Vice Versa

Table 1

List of reactions used in the metabolic model.

Name of reaction The reaction

PGI: G 6 P F 6 P
PFK: A T P + F 6 P A D P + F B P
ALD: F B P G 3 P + D H A P
TPI: D H A P G 3 P
G3PDH: P i O H + N A D + G 3 P N A D H + H + B P G
PGK: A D P + B P G A T P + 3 P G
PGM: 3 P G 2 P G
ENO:2 P G H 2 O + P E P
PyrK: A D P + P E P A T P + P y r
PyrD: N A D + P y r + C o A N A D H + H + A c C o A + C O 2
CitSY: H 2 O + A c C o A + O A A C o A + C i t
ACO: C i t i C i t
CitDH: N A D + i C i t N A D H + H + C O 2 + a K G A
AKGDH: N A D + C o A + a K G A N A D H + H + C O 2 + S u c C o A
SucCoASY: A D P + P i O H + S u c C o A A T P + C o A + S u c
SucDH: F A D + S u c F A D H 2 + F u m
FumHY: H 2 O + F u m M a l
MalDH: N A D + M a l N A D H + H + O A A
PEPCB: H 2 O + P E P + C O 2 P i O H + O A A
LacDH: N A D H + H + P y r N A D + L a c
PFLY: P y r + C o A A c C o A + F A
AcKNLR: A D P + P i O H + A c C o A A T P + C o A + A c
Resp: 1 . 3 3 A D P + 1 . 3 3 P i O H + N A D H + H + 0 . 5 O 2 1 . 3 3 A T P + N A D + 2 . 3 3 H 2 O
H 2 C O 3 S Y : H 2 O + C O 2 H 2 C O 3
G6PDH: N A D P + G 6 P N A D P H + H + 6 P G L
LAS: H 2 O + 6 P G L 6 P G
PGDH: N A D P + 6 P G N A D P H + H + C O 2 + R l 5 P
PPI: R l 5 P R 5 P
PPE: R l 5 P X u 5 P
TK1: R 5 P + X u 5 P G 3 P + S 7 P
TA: G 3 P + S 7 P F 6 P + E 4 P
TK2: X u 5 P + E 4 P F 6 P + G 3 P
PTS: G L C + P E P G 6 P + P y r
PPiOHHY: P P i O H + H 2 O 2 P i O H
GluDH: N A D P H + H + a K G A + N H 3 N A D P + H 2 O + G l u
GluLI: A T P + N H 3 + G l u A D P + P i O H + G l n
AspSY: A T P + H 2 O + A s p + G l n A M P + P P i O H + A s n + G l u
AspTA: O A A + G l u a K G A + A s p
AlaTA: P y r + G l u a K G A + A l a
ValAT: a K I V + G l u a K G A + V a l
LeuSYLR: N A D + H 2 O + A c C o A + a K I V + G l u N A D H + H + C o A + C O 2 + a K G A + L e u
aKIVSYLR: N A D P H + H + 2 P y r N A D P + H 2 O + C O 2 + a K I V
IleSYLR: N A D P H + H + P y r + G l u + T h r N A D P + H 2 O + C O 2 + a K G A + N H 3 + I l e
ProSYLR: A T P + 2 N A D P H + 2 H + G l u A D P + P i O H + 2 N A D P + H 2 O + P r o
SerLR: N A D + H 2 O + 3 P G + G l u P i O H + N A D H + H + a K G A + S e r
SerTHM: S e r + T H F H 2 O + G l y + M e T H F
H2SSYLR: 2 A T P + 3 N A D P H + T h i o r e d H 2 + 3 H + H 2 S O 4 A D P + P P i O H + 3 N A D P + T h i o r e d + 3 H 2 O
+ H 2 S + P A P
PAPNAS: H 2 O + P A P A M P + P i O H
CysSYLR: H 2 S + A c C o A + S e r C o A + C y s + A c
 PrppSY: A T P + R 5 P A M P + P R P P
HisSYLR: A T P + 2 N A D + 3 H 2 O + G l n + P R P P 2 P P i O H + P i O H + 2 N A D H + 2 H + a K G A + H i s + A I C A R
PheSYLR: G l u + C h o r H 2 O + C O 2 + a K G A + P h e
TyrSYLR: N A D + G l u + C h o r N A D H + H + C O 2 + a K G A + T y r
TrpSYLR: G l n + S e r + C h o r + P R P P P P i O H + 2 H 2 O + G 3 P + P y r + C O 2 + G l u + T r p
DhDoPHepAD: H 2 O + P E P + E 4 P P i O H + D a h p
DhqSY: D a h p P i O H + D h q
DhsSYLR: D h q H 2 O + D h s
ShiSY: N A D P H + H + D h s N A D P + S h i
ShiKN: A T P + S h i A D P + S h i 3 P
ChorSYLR: P E P + S h i 3 P 2 P i O H + C h o r
ThrSYLR: A T P + H 2 O + H S e r A D P + P i O H + T h r
MDAPSYLR: N A D P H + H + P y r + S u c C o A + G l u + A s p S A N A D P + C o A + a K G A + S u c + M D A P
LysSY: M D A P C O 2 + L y s
MetSYLR: H 2 O + S u c C o A + C y s + M T H F + H S e r P y r + C o A + S u c + N H 3 + M e t + T H F
AspSASY: A T P + N A D P H + H + A s p A D P + P i O H + N A D P + A s p S A
HSerDH: N A D P H + H + A s p S A N A D P + H S e r
CarPSY: 2 A T P + H 2 O + H 2 C O 3 + G l n 2 A D P + P i O H + G l u + C a r P
OrnSYLR: A T P + N A D P H + H + H 2 O + A c C o A + 2 G l u A D P + P i O H + N A D P + C o A + a K G A + O r n + A c
ArgSYLR: A T P + A s p + O r n + C a r P A M P + P P i O H + P i O H + F u m + A r g
ThioredRD: N A D P H + T h i o r e d + H N A D P + T h i o r e d H 2
H 2 O 2 o x : 2 H 2 O 2 2 H 2 O + O 2
FAD2NAD: N A D + F A D H 2 N A D H + F A D + H
AICARSYLR: 6 A T P + 3 H 2 O + C O 2 + A s p + 2 G l n + G l y + F A + P R P P 6 A D P + P P i O H + 6 P i O H + F u m
+ 2 G l u + A I C A R
IMPSYLR: F T H F + A I C A R H 2 O + T H F + I M P
AMPSYLR: A s p + G T P + I M P A M P + P i O H + F u m + G D P
AdKN: A T P + A M P 2 A D P
ADPRD: A D P + T h i o r e d H 2 T h i o r e d + H 2 O + d A D P
dADPKN: A T P + d A D P A D P + d A T P
IMPDH: N A D + H 2 O + I M P N A D H + H + X M P
GMPSY: A T P + H 2 O + G l n + X M P A M P + P P i O H + G l u + G M P
GuKN: A T P + G M P A D P + G D P
GDPKN: A T P + G D P A D P + G T P
GDPRD: T h i o r e d H 2 + G D P T h i o r e d + H 2 O + d G D P
dGDPKN: A T P + d G D P A D P + d G T P
UMPSYLR: O 2 + A s p + P R P P + C a r P P P i O H + P i O H + H 2 O + C O 2 + U M P + H 2 O 2
UrKN: A T P + U M P A D P + U D P
UDPKN: A T P + U D P A D P + U T P
CTPSY: A T P + H 2 O + G l n + U T P A D P + P i O H + G l u + C T P
CDPKN: A T P + C D P A D P + C T P
CMPKN: A T P + C M P A D P + C D P
CDPRD: T h i o r e d H 2 + C D P T h i o r e d + H 2 O + d C D P
dCDPKN: A T P + d C D P A D P + d C T P
UDPRD: T h i o r e d H 2 + U D P T h i o r e d + H 2 O + d U D P
dUDPKN: A T P + d U D P A D P + d U T P
dUTPPPAS: H 2 O + d U T P P P i O H + d U M P
dTMPSY: M e T H F + d U M P D H F + d T M P
dTMPKN: A T P + d T M P A D P + d T D P
dTDPKN: A T P + d T D P A D P + d T T P
DHFRD: N A D P H + H + D H F N A D P + T H F
FTHFSYLR: N A D P + H 2 O + M e T H F N A D P H + H + F T H F
GlyCA: N A D + G l y + T H F N A D H + H + C O 2 + N H 3 + M e T H F
MeTHFRD: N A D H + H + M e T H F N A D + M T H F
AcCoACB: A T P + H 2 O + A c C o A + C O 2 A D P + P i O H + M a l C o A
MalCoATA: M a l C o A + A C P C o A + M a l A C P
AcACPSY: M a l A C P C O 2 + A c A C P
C120SY: 1 0 N A D P H + 1 0 H + A c A C P + 5 M a l A C P 1 0 N A D P + 5 H 2 O + 5 C O 2 + C 1 2 0 A C P + 5 A C P
C140SY: 1 2 N A D P H + 1 2 H + A c A C P + 6 M a l A C P 1 2 N A D P + 6 H 2 O + 6 C O 2 + C 1 4 0 A C P + 6 A C P
C160SY: 1 4 N A D P H + 1 4 H + A c A C P + 7 M a l A C P 1 4 N A D P + 7 H 2 O + 7 C O 2 + C 1 6 0 A C P + 7 A C P
C181SY: 1 5 N A D P H + 1 5 H + A c A C P + 8 M a l A C P 1 5 N A D P + 8 H 2 O + 8 C O 2 + C 1 8 1 A C P + 8 A C P
AcylTF: C 1 6 0 A C P + C 1 8 1 A C P + G o 3 P 2 A C P + P A
Go3PDH: N A D P H + H + D H A P N A D P + G o 3 P
DGoKN: A T P + D G o A D P + P A
CDPDGoSY: C T P + P A P P i O H + C D P D G o
PSerSY: S e r + C D P D G o C M P + P S e r
PSerDC: P S e r C O 2 + P E t h A n
GlnF6PTA: F 6 P + G l n G l u + G A 6 P
GlcAnMU: G A 6 P G A 1 P
NAGUrTF: A c C o A + U T P + G A 1 P P P i O H + C o A + U D P N A G
LipaSYLR: A T P + 2 C M P K D O + 2 U D P N A G + C 1 2 0 A C P + 5 C 1 4 0 A C P A D P + 2 C M P + U M P + U D P
+ 6 A C P + L i p a + 2 A c
A5PIR: R l 5 P A r 5 P
PGLCMT: G 6 P G 1 P
CMPKDOSYLR: 2 H 2 O + P E P + A r 5 P + C T P P P i O H + 2 P i O H + C M P K D O
ADPHEPSY: A T P + S 7 P P P i O H + A D P H E P
UDPGlcSY: G 1 P + U T P P P i O H + U D P G l c
EthANPT: C M P + P E t h A n C D P E t h A n + D G o
LpsSYLR: 3 A D P H E P + 3 C M P K D O + 2 U D P G l c + L i p a + 2 C D P E t h A n 3 A D P + 3 C M P + 2 C D P + 2 U D P + L p s
PGSYLR: G o 3 P + C D P D G o P i O H + C M P + P G
CLSY: P G + C D P D G o C M P + C L
PeptidoSYLR: 5 A T P + N A D P H + H + P E P + 3 A l a + M D A P + 2 U D P N A G 5 A D P + 7 P i O H + N A D P + U M P +
U D P + P e p t i d o
GlcgSY: A T P + G 1 P A D P + P P i O H + G l c g
ATPHY: A T P + H 2 O A D P P i O H
DNASYLR: 2 H 2 O + 0 . 2 4 6 d A T P + 0 . 2 5 4 d G T P + 0 . 2 5 4 d C T P + 0 . 2 4 6 d T T P 2 P i O H + D N A
RNASYLR: 0 . 2 6 2 A T P + 2 H 2 O + 0 . 3 2 2 G T P + 0 . 2 C T P + 0 . 2 1 6 U T P 2 P i O H + R N A
ProtSYLR: 2 A T P + 3 H 2 O + 0 . 0 9 6 1 A l a + 0 . 0 5 5 0 6 A r g + 0 . 0 4 5 0 5 A s n + 0 . 0 4 5 0 5 A s p + 0 . 0 1 7 0 2 C y s + 0 . 0 4 9 0 5 G l n + 0 . 0 4 9 0 5 G l u + 0 . 1 1 5 1 G l y + 0 . 0 1 8 0 2 H i s + 0 . 0 5 4 0 5 I l e + 0 . 0 8 4 0 8 L e u + 0 . 0 6 4 0 6 L y s + 0 . 0 2 9 0 3 M e t + 0 . 0 3 5 0 4 P h e + 0 . 0 4 1 0 4 P r o + 0 . 0 4 0 0 4 S e r + 0 . 0 4 7 0 5 T h r + 0 . 0 1 1 0 1 T r p + 0 . 0 2 6 0 3 T y r + 0 . 0 7 9 0 8 V a l + 2 G T P 2 A D P + 4 P i O H + 2 G D P + P r o t
LipidSYLR: 0 . 0 2 6 6 C L + 0 . 2 0 2 P G + 0 . 7 7 1 4 P E t h A n L i p i d
BiomSYLR: 0 . 0 0 4 5 6 1 G l c g + 0 . 0 0 0 2 6 6 3 L p s + 0 . 0 0 0 8 9 3 3 P e p t i d o + 0 . 0 0 2 2 9 1 D N A + 0 . 0 1 4 4 6 R N A + 0 . 1 2 2 7 P r o t + 0 . 0 0 3 6 4 2 L i p i d B i o m