Dynamic Metabolic Flux Analysis Demonstrated on Cultures Where the Limiting Substrate Is Changed from Carbon to Nitrogen and Vice Versa

Table 3

Parameters for the polynomial fitting of the different time series. 𝑊₁ is expressed in fraction of the time between the first measurement and the last measurement. 𝑊₂ is expressed as a fraction of 𝑊₁. PD is the highest degree a polynomial may have; an L is displayed if the data were fitted to a logistic curve.

	C→N limitation			N→C limitation
	𝑊1	𝑊2	PD	𝑊1	𝑊2	PD
GLC	0.40	0.87	5	0.35	0.45	3
NH3	0.50	0.80	2	1	1	L
PiOH	0.70	0.75	5	0.45	0.80	4
O2	0.18	0.90	7	0.11	0.90	7
CO2	0.12	0.90	4	0.12	0.90	7
Ac	0.70	0.88	5	0.15	0.70	3
Lac	1	1	7	0.41	0.80	7
Pyr	0.40	0.80	6	1	1	L
Suc	0.40	0.80	6	0.82	0.99	5
Biomass	0.50	0.70	8	0.37	0.80	4