Ordered Assembly of the Adhesive and Electrochemical Connections within Newly Formed Intercalated Disks in Primary Cultures of Adult Rat Cardiomyocytes
Figure 4
Co-localization of PKP2 with N-cadherin and desmocollin in newly formed cell-cell contacts. ARCs were immunolabeled with antibodies to PKP2 (green: a, b, d, e) and pan-cadherin (red: a, c) or desmocollin (red: d, f) on day 5 post plating. There is an extensive overlap in localization of PKP2 with both N-cadherin and desmocollin. Note that there was a localization of N-cadherin along the lateral aspects of cellular projections at the cell-contact sites (arrows in (a)–(c)). These may represent filopodial extensions that mature into cell-adhesion domains. There was also noted to be some regional variation in the relative amounts of PKP2 and desmocollin (arrows in (d)–(f)). Scale bars are 10 m.
