Short-Term Evaluation in Growing Rats of Diet Containing Bacillus thuringiensis Cry1Ia12 Entomotoxin: Nutritional Responses and Some Safety Aspects

Table 1

Composition (g/kg) of the NPC, EW, and experimental diets^∗.

Ingredients		Diets
	NPC	EW	Cry1Ia12
Maize starch	475	342.55	342.55
Glucose	150	150	150
Maize oil	150	150	150
Vitamin mix^∗∗	50	50	50
Mineral mix^∗∗∗	50	50	50
Neofibre^∗∗∗	125	125	125
Egg White (75.5%)	—	132.45	132.35
Cry1Ia12	—	—	0.1

∗Cry1Ia12: Cry1Ia12 protein; NPC: nonprotein control; EW: egg-white protein.
∗∗Vitamin mix (g/kg): vitamin B₁₂ (100%), 0.02; folic acid, 0.04; biotin (1%), 4.0; pyridoxine HCl, 0.04; thiamine HCl, 0.06; riboflavin (99%), 0.21; Ca-pantothenate (45%), 1.2; nicotinic acid, 4.0; inositol, 4.0; p-amino-benzoic acid, 12.0; choline chloride (50%), 24.0; maize starch, 950.43.
∗∗∗Mineral mix (g/kg): calcium citrate, 296.1; calcium carbonate (40%), 65.8; copper carbonate, 1.1; magnesium carbonate, 34.3; zinc carbonate, 0.48; ferric citrate, 9.1; magnesium chloride⋅6H₂O, 5.82; sodium chloride, 74.0; potassium chloride, 119.5; monobasic calcium phosphate, 108.2; dibasic potassium phosphate, 210.1; sodium fluoride, 0.48; potassium iodate, 0.1; magnesium sulphate, 75.4.
∗∗∗∗Neofibre (Nuteral, 80% fibre, Fortaleza, Brazil).