Figure 5: Effect of 𝛽 -glu6 on the CD8+/IFN- 𝛾 + T cells induced by pB144 in different organs. Mice were immunized with pB144 and treated with 𝛽 -glu6, and boosted at day 30. Ten days after boosting, the liver lymphocytes, PBMC, and splenocytes were isolated and cultured for 5 hours in the absence or in the presence of a specific peptide corresponding to K b -restricted CTL epitope of HBcAg. Cells were then double-stained with PE-anti-CD8a and APC-anti-IFN- 𝛾 antibodies and analyzed cytofluorometrically. Data are expressed as mean ± SD of 3 mice/group. * 𝑃 < . 0 5 , pB144+G versus pB144 alone.