𝛽-Glucan Oligosaccharide Enhances CD𝟖⁺ T Cells Immune Response Induced by a DNA Vaccine Encoding Hepatitis B Virus Core Antigen

Table 1

Effect of 𝛽-glu6 on pB144-induced antibody production*.

Immunogens	Anti-HBcAg antibody titer (Log2 ± SD)**
	Week 4 after priming			Week 2 after boosting
	IgG	IgG1	IgG2a	IgG	IgG1	IgG2a
G	N.D. ***	N.D.	N.D.	N.D.	N.D.	N.D.
pB144	9.81±0.21	N.D.	N.D.	12.98±0.18	N.D.	11.81±0.55
pB144+G	11.40±0.09 ****	N.D.	6.90±0.49	14.57±0.50 ****	N.D.	12.13±0.6
PBS	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.

*The sera from immunized mice were collected 4 weeks after priming and 2 weeks after boosting. Anti-HBcAg IgG, IgG1, and IgG2a were analyzed by ELISA. The initial dilution of each serum from immunized mouse was 1 : 100 and followed with serial of three-fold dilution for anti-HBc IgG detection, and two-fold dilution for anti-HBc IgG1, IgG2a analysis.
**The well with an absorbance OD₄₅₀ ≥ 0.20 (the blank well, ~0.020) was scored as positive. The anti-HBc antibody titers are expressed as the reciprocal of the highest dilution showing a positive reaction and calculated as the mean ± standard deviation (SD) of Log₂ for each group (6 mice/group).
***N.D. indicates that anti-HBc antibody in the sera of the mice is under detection level.
**** 𝑃<.05, pB144 +G versus pB144 alone.