Telomere Attrition Occurs during Ex Vivo Expansion of Human Dental Pulp Stem Cells
Figure 5
Osteogenic and chondrogenic potential of DPSCs. (a) Phase contrast micrograph of osteogenic nodule after 4 week-cultivation in differentiation medium. (b) Histological examination revealed multilayered structure consisting of osteoblast-like cells surrounded by extracellular matrix containing collagen fibres; Ladewig blue trichrome. (c) Immunoperoxidase histochemistry of osteonectin confirmed the presence of this bone-specific glycoprotein that links minerals to collagen within the micromass pellets. (d) Chondrogenic micromass bodies were composed of cells and extracellular matrix. Alcian blue staining confirmed the presence of sulphated acidic glycosaminoglycans, a principal component of cartilaginous ground substance. Scale bars (a)-(b) 100 m, (c) 200 m, (d) and 50 m.
