Combination of Intensive Chemotherapy and Anticancer Vaccines in the Treatment of Human Malignancies: The Hematological Experience

Table 1

Cryopreservation of autologous peripheral blood stem cell grafts in cancer patients; a summary of the effects on immunocompetent cells when grafts were prepared with 2%, 4%, 5%, and 10% DMSO.

T cell population	Functional characteristics	Effects of cryopreservation with DMSO at various concentrations
Major T cell subsets
CD3^+CD4^+	T helper	No effect of different DMSO levels
CD3^+CD8^+	Cytotoxic	No effect of different DMSO levels
CD3^+CD56^+CD16^+	NK T cells	Highest viability with 2% DMSO
CD3^+CD56^+CD16^-	NK T cells	Highest viability with 2% DMSO
CD62L-defined subsets
CD4^+CD62^+, CD8^+CD62^+	Naive and central memory	Highest viability when using DMSO 5%
CD4^+CD62^low, CD8^+CD62^low	Late effector T cells	CD4^+ cells show highest viability with DMSO 2% and 4%
CD4^+CD62^-, CD8^+CD62^-	Effector memory	No effect of different DMSO levels
Subsets defined by chemokine receptor expression
CD4^+CCR7^+, CD8^+CCR7^+	Naive and central memory T cells	The CD4^+ cells showed decreased viability when using 10% DMSO
CD3^+CD45^+CCR4^-CCR6^-		Decreased viability when using 2% DMSO
Other CCR2, CCR4, CCR7		No effect of different DMSO levels
defined T cell subsets
CD4^+CD25^+FoxP3^+ T cells	Natural regulatory T cells	Decreased viability when using 10% DMSO

Autografts were prepared for cancer patients after mobilisation with chemotherapy plus G-CSF. After the aphereses cell concentrations were adjusted and cells stored in nitrogen for 5-6 years as described in the text [50].