BioMed Research International / 2010 / Article / Fig 4

Research Article

Phenotypic Transition of the Collecting Duct Epithelium in Congenital Urinary Tract Obstruction

Figure 4

E-cadherin and ๐›ผ -smooth muscle actin immunoreactivity. Normal kidneys ((a)โ€“(c)). In the normal mid gestation (18 weeks) (a), late gestation (27 weeks) (b), and postnatal (12 months) (c) kidneys, ๐›ผ -smooth muscle actin ( ๐›ผ -SMA)-IR (green) was absent in the CD epithelium; however, intercellular localization of E-cadherin-IR (red) was well established from mid gestation onwards (arrows). Obstructed kidneys ((d)โ€“(f)). Circumferential collars expressing ๐›ผ -SMA-IR (green) are present in the (d) mid gestation (18 weeks), (e) late gestation (36 weeks), and (f) postnatal (16 months) kidneys (arrows). De novo expression of ๐›ผ -SMA-IR in the obstructed CD epithelium is present at 18 weeks gestation ((d) inset, arrowhead) but not in the late gestation (e) or postnatal (f) kidneys. As in Figure 3, E-cadherin expression (red) is disrupted, with translocation from the cell membrane to cytoplasm ((d)โ€“(f)) and to the nucleus ((e) inset, arrowhead). Scale bar = 25โ€‰ ๐œ‡ m.

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