706872.fig.002
Figure 2: Detection of alpha-thalassemia on agarose gel after multiplex PCR. Lane 1: DNA blank; lane 2: normal controls ( 𝛼 𝛼 / 𝛼 𝛼 ) with a 2350 bp LIS1 internal control band and a 1800 bp α2-globin gene band; lane 3: single 𝛼 -globin gene deletion carrier ( 𝛼 3 . 7 / 𝛼 𝛼 ) with an internal control band, a 2022 bp deletion-specific band and an α2-globin gene band; lane 4: individual homozygous for 𝛼 -globin gene deletion ( 𝛼 3 . 7 / 𝛼 3 . 7 ) with an internal control band and a 2022 bp deletion-specific band; lane 5 : 500 bp molecular weight marker.