High Prevalence of Alpha- and Beta-Thalassemia in the Kadazandusuns in East Malaysia: Challenges in Providing Effective Health Care for an Indigenous Group

Figure 2

Detection of alpha-thalassemia on agarose gel after multiplex PCR. Lane 1: DNA blank; lane 2: normal controls (𝛼𝛼/𝛼𝛼) with a 2350 bp LIS₁ internal control band and a 1800 bp α2-globin gene band; lane 3: single 𝛼-globin gene deletion carrier (−𝛼^3.7/𝛼𝛼) with an internal control band, a 2022 bp deletion-specific band and an α2-globin gene band; lane 4: individual homozygous for 𝛼-globin gene deletion (−𝛼^3.7/−𝛼^3.7) with an internal control band and a 2022 bp deletion-specific band; lane 5 : 500 bp molecular weight marker.