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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 716515, 9 pages
http://dx.doi.org/10.1155/2010/716515
Research Article

A Novel Lectin with Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Dried Fruiting Bodies of the Monkey Head Mushroom Hericium erinaceum

1State Key Laboratory for Agrobiotechnology, Department of Microbiology, China Agricultural University, Beijing 100193, China
2School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong

Received 4 March 2010; Revised 14 April 2010; Accepted 14 April 2010

Academic Editor: Stavros J. Hamodrakas

Copyright © 2010 Yanrui Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A lectin designated as Hericium erinaceum agglutinin (HEA) was isolated from dried fruiting bodies of the mushroom Hericium erinaceum with a chromatographic procedure which entailed DEAE-cellulose, CM-cellulose, Q-Sepharose, and FPLC Superdex 75. Its molecular mass was estimated to be 51 kDa and its N-terminal amino acid sequences was distinctly different from those of other isolated mushroom lectins. The hemagglutinating activity of HEA was inhibited at the minimum concentration of 12.5 mM by inulin. The lectin was stable at pH 1.9–12.1 and at temperatures up to 7 0 C, but was inhibited by H g 2 + , C u 2 + , and F e 3 + ions. The lectin exhibited potent mitogenic activity toward mouse splenocytes, and demonstrated antiproliferative activity toward hepatoma (HepG2) and breast cancer (MCF7) cells with an I C 5 0 of 56.1  𝜇 M and 76.5  𝜇 M, respectively. It manifested HIV-1 reverse transcriptase inhibitory activity with an I C 5 0 of 31.7  𝜇 M. The lectin exhibited potent mitogenic activity toward murine splenocytes but was devoid of antifungal activity.