BioMed Research International

Research Article

A Novel Lectin with Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Dried Fruiting Bodies of the Monkey Head Mushroom Hericium erinaceum

Table 6

Comparison of characteristics of HEL [17] and HEA (this study).
CharacteristicsHELHEA
Chromatographic behavior on
    DEAE ion exchangernot determinedadsorbed, eluted with 300 mM NaCl
    DEAE-Toyopearl columnadosorbed, eluted with 200 mM NaCl in Tris buffer.not determined
    CM ion exchangernot determinedadsorbed, eluted with 50 Mm NaCl
    Mono-S columnAbsorbednot determined
Moleculr Mass (kDa)5451
Subunit molecular mass (kDa)15 and 1651
N-terminal sequencenot determinedAFGQLSFANLAAADF
ThermostabilityUp to 7 0 ∘ C up to 7 0 ∘ C
pH stability5.0–10.51.9–12.1
Sugar specificity
    (i) mono- or oligo-saccharidesNeuGc, NeuAc, 3 -N-Acetylneuramin-lactose and galacturonic acidinulin and not by simple sugars
    (ii) glycoproteinsasialo-BSM, Asialofetuinnot determined
Sialic acid-binding activatingYesnot determined
Effect of cations on hemagglutinating activityno effectactivity inhibited by Cu2+, Fe3+, and Hg2+ ions
Antifungal activitynot determinedno effect
Antiproliferative activitynot determinedIC50 of 56.1  𝜇 M toward HepG2 cells, and 76.5  𝜇 M toward MCF7 cells, respectively
Mitogenic activity toward splenocytesnot determined35.8 fold increase over basal value
HIV-1 reverse transcriptase inhibitory activitynot determined I C 5 0 = 3 1 . 7 𝜇 M