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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 721485, 10 pages
http://dx.doi.org/10.1155/2010/721485
Research Article

Aberrant Methylation of Thrombospondin-1 and Its Association with Reduced Expression in Gastric Cardia Adenocarcinoma

1Department of Laboratory of Pathology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China
2Department of Cardiothoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China
3Department of Laboratory of Flow Cytometry of Hebei Cancer Institute, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China

Received 27 August 2009; Revised 8 December 2009; Accepted 31 December 2009

Academic Editor: Haodong Xu

Copyright © 2010 Wei Guo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. N. L. Baenziger, G. N. Brodie, and P. W. Majerus, “A thrombin-sensitive protein of human platelet membranes,” Proceedings of the National Academy of Sciences of the United States of America, vol. 68, no. 1, pp. 240–243, 1971. View at Google Scholar · View at Scopus
  2. P. G. Robey, M. F. Young, L. W. Fisher, and T. D. McClain, “Thrombospondin is an osteoblast-derived component of mineralized extracellular matrix,” Journal of Cell Biology, vol. 108, no. 2, pp. 719–727, 1989. View at Google Scholar · View at Scopus
  3. E. A. Jaffe, J. T. Ruggiero, and D. J. Falcone, “Monocytes and macrophages synthesize and secrete thrombospondin,” Blood, vol. 65, no. 1, pp. 79–84, 1985. View at Google Scholar · View at Scopus
  4. R. A. Majack, S. C. Cook, and P. Bornstein, “Platelet-derived growth factor and heparin-like glycosaminoglycans regulate thrombospondin synthesis and deposition in the matrix by smooth muscle cells,” Journal of Cell Biology, vol. 101, no. 3, pp. 1059–1070, 1985. View at Google Scholar · View at Scopus
  5. J. C. Adams and J. Lawler, “The thrombospondins,” International Journal of Biochemistry and Cell Biology, vol. 36, no. 6, pp. 961–968, 2004. View at Publisher · View at Google Scholar · View at Scopus
  6. H. Chen, M. E. Herndon, and J. Lawler, “The cell biology of thrombospondin-1,” Matrix Biology, vol. 19, no. 7, pp. 597–614, 2000. View at Publisher · View at Google Scholar · View at Scopus
  7. Z. Li, M. J. Calzada, J. M. Sipes et al., “Interactions of thrombospondins with a4ß1 integrin and CD47 differentially modulate T cell behavior,” Journal of Cell Biology, vol. 157, no. 3, pp. 509–519, 2002. View at Publisher · View at Google Scholar · View at Scopus
  8. S. Schultz-Cherry and J. E. Murphy-Ullrich, “Thrombospondin causes activation of latent transforming growth factor-β secreted by endothelial cells by a novel mechanism,” Journal of Cell Biology, vol. 122, no. 4, pp. 923–932, 1993. View at Google Scholar · View at Scopus
  9. S. S. Li, Z. Liu, M. Uzunel, and K.-G. Sundqvist, “Endogenous thrombospondin-1 is a cell-surface ligand for regulation of integrin-dependent T-lymphocyte adhesion,” Blood, vol. 108, no. 9, pp. 3112–3120, 2006. View at Publisher · View at Google Scholar · View at Scopus
  10. L. Lamy, A. Foussat, E. J. Brown, P. Bornstein, M. Ticchioni, and A. Bernard, “Interactions between CD47 and thrombospondin reduce inflammation,” Journal of Immunology, vol. 178, no. 9, pp. 5930–5939, 2007. View at Google Scholar · View at Scopus
  11. Z. Li, L. He, K. E. Wilson, and D. D. Roberts, “Thrombospondin-1 inhibits TCR-mediated T lymphocyte early activation,” Journal of Immunology, vol. 166, no. 4, pp. 2427–2436, 2001. View at Google Scholar · View at Scopus
  12. M. Sarfati, G. Fortin, M. Raymond, and S. Susin, “CD47 in the immune response: role of thrombospondin and SIRP-alpha reverse signaling,” Current Drug Targets, vol. 9, no. 10, pp. 842–850, 2008. View at Google Scholar · View at Scopus
  13. A. N. Vallejo, L. O. Mügge, P. A. Klimiuk, C. M. Weyand, and J. J. Goronzy, “Central role of thrombospondin-1 in the activation and clonal expansion of inflammatory T cells,” Journal of Immunology, vol. 164, no. 6, pp. 2947–2954, 2000. View at Google Scholar · View at Scopus
  14. B. Sid, H. Sartelet, G. Bellon et al., “Thrombospondin 1: a multifunctional protein implicated in the regulation of tumor growth,” Critical Reviews in Oncology/Hematology, vol. 49, no. 3, pp. 245–258, 2004. View at Publisher · View at Google Scholar · View at Scopus
  15. D. D. Roberts, “Regulation of tumor growth and metastasis by thrombospondin-1,” FASEB Journal, vol. 10, no. 10, pp. 1183–1191, 1996. View at Google Scholar · View at Scopus
  16. A. E. Canfield and A. M. Schor, “Evidence that tenascin and thrombospondin-1 modulate sprouting of endothelial cells,” Journal of Cell Science, vol. 108, no. 2, pp. 797–809, 1995. View at Google Scholar · View at Scopus
  17. D. L. Weinstat-Saslow, V. S. Zabrenetzky, K. VanHoutte, W. A. Frazier, D. D. Roberts, and P. S. Steeg, “Transfection of thrombospondin 1 complementary DNA into a human breast carcinoma cell line reduces primary tumor growth, metastatic potential, and angiogenesis,” Cancer Research, vol. 54, no. 24, pp. 6504–6511, 1994. View at Google Scholar · View at Scopus
  18. V. Zabrenetzky, C. C. Harris, P. S. Steeg, and D. D. Roberts, “Expression of the extracellular matrix molecule thrombospondin inversely correlates with malignant progression in melanoma, lung and breast carcinoma,” International Journal of Cancer, vol. 59, no. 2, pp. 191–195, 1994. View at Publisher · View at Google Scholar · View at Scopus
  19. G. Martin-Manso, S. Galli, L. A. Ridnour, M. Tsokos, D. A. Wink, and D. D. Roberts, “Thrombospondin 1 promotes tumor macrophage recruitment and enhances tumor cell cytotoxicity of differentiated U937 cells,” Cancer Research, vol. 68, no. 17, pp. 7090–7099, 2008. View at Publisher · View at Google Scholar · View at Scopus
  20. V. P. Castle, X. Ou, K. O'Rourke, and V. M. Dixit, “High level thrombospondin 1 expression in two NIH 3T3 cloned lines confers serum- and anchorage-independent growth,” Journal of Biological Chemistry, vol. 268, no. 4, pp. 2899–2903, 1993. View at Google Scholar · View at Scopus
  21. N. Oue, S. Matsumura, H. Nakayama et al., “Reduced expression of the TSP1 gene and its association with promoter hypermethylation in gastric carcinoma,” Oncology, vol. 64, no. 4, pp. 423–429, 2003. View at Publisher · View at Google Scholar · View at Scopus
  22. A. Rojas, S. Meherem, Y.-H. Kim et al., “The aberrant methylation of TSP1 suppresses TGF-ß1 activation in colorectal cancer,” International Journal of Cancer, vol. 123, no. 1, pp. 14–21, 2008. View at Publisher · View at Google Scholar · View at Scopus
  23. Y. Yokokawa, S. Ohta, J. Hou et al., “Ecological study on the risks of esophageal cancer in Ci-Xian, China: the importance of nutritional status and the use of well water,” International Journal of Cancer, vol. 83, no. 5, pp. 620–624, 1999. View at Publisher · View at Google Scholar · View at Scopus
  24. J. R. Siewert and H. J. Stein, “Classification of adenocarcinoma of the oesophagogastric junction,” British Journal of Surgery, vol. 85, no. 11, pp. 1457–1459, 1998. View at Publisher · View at Google Scholar · View at Scopus
  25. J. G. Herman, J. R. Graff, S. Myöhänen, B. D. Nelkin, and S. B. Baylin, “Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands,” Proceedings of the National Academy of Sciences of the United States of America, vol. 93, no. 18, pp. 9821–9826, 1996. View at Publisher · View at Google Scholar · View at Scopus
  26. J.-Y. Li, A. B. Ershow, Z.-J. Chen et al., “A case-control study of cancer of the esophagus and gastric cardia in Linxian,” International Journal of Cancer, vol. 43, no. 5, pp. 755–761, 1989. View at Google Scholar · View at Scopus
  27. Q.-W. Yang, S. Liu, Y. Tian et al., “Methylation-associated silencing of the thrombospondin-1 gene in human neuroblastoma,” Cancer Research, vol. 63, no. 19, pp. 6299–6310, 2003. View at Google Scholar · View at Scopus
  28. S. Lee, K. S. Hwang, H. J. Lee, J.-S. Kim, and G. H. Kang, “Aberrant CpG island hypermethylation of multiple genes in colorectal neoplasia,” Laboratory Investigation, vol. 84, no. 7, pp. 884–893, 2004. View at Publisher · View at Google Scholar · View at Scopus
  29. H. C. Kim, J. C. Kim, S. A. Roh et al., “Aberrant CpG island methylation in early-onset sporadic gastric carcinoma,” Journal of Cancer Research and Clinical Oncology, vol. 131, no. 11, pp. 733–740, 2005. View at Publisher · View at Google Scholar · View at Scopus
  30. B. Klump, C.-J. Hsieh, K. Holzmann, M. Gregor, and R. Porschen, “Hypermethylation of the CDKN2/p16 promoter during neoplastic progression in Barrett's esophagus,” Gastroenterology, vol. 115, no. 6, pp. 1381–1386, 1998. View at Google Scholar · View at Scopus
  31. S. A. Foster, D. J. Wong, M. T. Barrett, and D. A. Galloway, “Inactivation of p16 in human mammary epithelial cells by CpG island methylation,” Molecular and Cellular Biology, vol. 18, no. 4, pp. 1793–1801, 1998. View at Google Scholar · View at Scopus
  32. A. Bird, “Molecular biology. Methylation talk between histones and DNA,” Science, vol. 294, no. 5549, pp. 2113–2115, 2001. View at Publisher · View at Google Scholar · View at Scopus
  33. M. O. Li and R. A. Flavell, “TGF-β: a master of all T cell trades,” Cell, vol. 134, no. 3, pp. 392–404, 2008. View at Publisher · View at Google Scholar · View at Scopus
  34. J. P. Annes, J. S. Munger, and D. B. Rifkin, “Making sense of latent TGFβ activation,” Journal of Cell Science, vol. 116, no. 2, pp. 217–224, 2003. View at Publisher · View at Google Scholar · View at Scopus
  35. K. Nakamura, A. Kitani, and W. Strober, “Cell contact-dependent immunosuppression by CD4+CD25+ regulatory T cells is mediated by cell surface-bound transforming growth factor β,” Journal of Experimental Medicine, vol. 194, no. 5, pp. 629–644, 2001. View at Publisher · View at Google Scholar · View at Scopus
  36. P. Grimbert, S. Bouguermouh, N. Baba et al., “Thrombospondin/CD47 interaction: a pathway to generate regulatory T cells-from human CD4+CD25- T cells in response to inflammation,” Journal of Immunology, vol. 177, no. 6, pp. 3534–3541, 2006. View at Google Scholar · View at Scopus