Mechanism of Catch Force: Tethering of Thick and Thin Filaments by Twitchin

Figure 2

Time course of release of catch force and corresponding incorporation of ³³𝑃 into proteins of permeabilized ABRM following cAMP treatment and photolysis of caged ³³𝑃-ATP. Muscles were frozen at times indicated after photolysis. Labeled proteins are shown by the following symbols: □, ∼45 kDa; ○, ∼100 kDa; ∆, ∼200 kDa; , high-molecular-weight protein. 𝑇 = 20 C. Reproduced from [58] with permission.