Review Article

Mechanism of Catch Force: Tethering of Thick and Thin Filaments by Twitchin

Figure 3

Protein phosphorylation under different mechanical conditions in permeabilized ABRM. Muscles were incubated with 3 2 𝑃 -ATP starting in an initial relaxing solution and then either frozen (a) after 4 min in relaxing solution, (b) after 1 minute in relaxing solution and 3 minutes in activation solution (pCa5), (c) treatment as in (b), plus 1 minute in relaxing solution (catch), and (d) after treatment as in (b), plus 1 min in relaxing solution containing cAMP (release of catch). Shown are a Coomassie Blue-stained 4%–15% acrylamide gradient gel and corresponding phosphorimager autoradiogram. 𝑇 = 20 C. Reproduced from [58] with permission.
725207.fig.003