Research Article

Identification of Four Entamoeba histolytica Organellar DNA Polymerases of the Family B and Cellular Localization of the Ehodp1 Gene and EhODP1 Protein

Figure 3

Alignment of the conserved boxes identified in EhODP1, DNA polymerases encoded in fungi mitochondrial plasmids and in a T. vaginalis protein. (a) Amino acid sequences corresponding to boxes I, II and III which characterize the family B of DNA polymerases. (b) Amino acid sequences of boxes A and B conserved in the aligned polypeptides that were found in this work. Identical amino acids are shown in black. Conserved changes are shown in grey. Numbers correspond to the first and last residues in conserved boxes for each polypeptide. Arrows indicate the aspartic acid residues that are required for the catalytic activity of DNA polymerases. Asterisks indicate sequences corresponding to EhODPs. Nin: Neurospora intermedia; Gsp: Gelasinospora sp; Aim: Ascobolus immersus; Fve: Flammulina velutipes; Cpe: Crinipellis perniciosa; Aae: Agrocybe aegerita; Kla-2: Kluyveromyces lactis DNA polymerase 2, found in plasmid pGKL-2; Skl: Saccharomyces kluyveri; Pet: Pichia etchellsii; Mco: Morchella conica; Ppu: Porphyra purpurea; Pan: Podospora anserina; Kla-1: Kluyveromyces lactis DNA polymerase 1, found in plasmid pGKL-1; Pkl: Pichia kluyveri; Ehi-1: E. histolytica organellar DNA polymerase 1; Ehi-2: E. histolytica organellar DNA polymerase 2; Ehi-3: E. histolytica organellar DNA polymerase 3; Ehi-4: E. histolytica organellar DNA polymerase 4; Tva: T. vaginalis.
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