Identification of Four Entamoeba histolytica Organellar DNA Polymerases of the Family B and Cellular Localization of the Ehodp1 Gene and EhODP1 Protein
Figure 8
Subcellular localization of EhODP1 in EhkOs using anti-rEhODP1-168 antibodies and laser confocal microscopy. Fixed and permeabilized E. histolytica trophozoites were incubated with anti-rEhODP1-168 antibodies and fluorescein-labeled goat anti-rat antibodies, stained with PI and observed through a laser confocal microscope. (a) Immunolocalization of EhODP1 in EhkOs. (b) Negative control performed with preimmune serum. (PI) Cells stained with propidium iodide (red channel). (-rEhODP1) EhODP1 protein immunolocalized in EhkOs with anti-rEhODP1-168 antibodies (green channel). (M) Merging of red and green fluorescent signals. Arrows indicate EhkOs. Squares show an image amplification of a cytoplasmic DNA-containing structure. The bar scale corresponds to 8 m.
