Nonviral Gene Delivery to Mesenchymal Stem Cells Using Cationic Liposomes for Gene and Cell Therapy

Figure 3

Evaluation of GFP expression along 7 days after transfection of MSC with Lipoplexes (a, c) and percentage of cell viability and cell Recovery of the different liposomal formulations measured 24 hours after transfection (b, d). Variation of liposome amount, maintaining DNA amount (2 𝜇g) at charge ratios L/D 1.25 and 2, using 2.5 𝜇L and 4 𝜇L of LF2000, respectively (a, b). Variation of DNA amount, maintaining liposome volume (2.5 𝜇L) at charge ratios L/D 1.25 and 2.5, using 2 𝜇g and 1 𝜇g of DNA, respectively (c, d). The data was obtained from the average of two independent experiments with duplicates±SEM.

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