Nonviral Gene Delivery to Mesenchymal Stem Cells Using Cationic Liposomes for Gene and Cell Therapy

Table 2

Immunophenotype evaluation of transfected MSC through flow cytometry analysis. The identification of MSC characteristic phenotype was assessed by fluorescent-conjugated monoclonal antibodies against CD73, CD105, and CD90 receptors. Controls with non-transfected cells were also performed as well as the appropriate isotype controls IgG 𝛾1 and IgG 𝛾2.

Phenotype marker	CD73^+	CD105^+	CD90^+
MSC (control)	96.5%	95.3%	92.1%
Transfected MSC	89.3%	87.0%	89.8%
GFP^+ MSC	83.0%	74.2%	86.5%