Depletion of DNMT3A Suppressed Cell Proliferation and Restored PTEN in Hepatocellular Carcinoma Cell

Table 2

Primers sequences for MSP and bisulfite sequencing analysis.

Gene	Primers (5^-3^)	Anneal temperature (^∘C)
𝑃𝑇𝐸𝑁-M^a	Forward: TTTTTTTTCGGTTTTTCGAGGC	59
	Reverse: CAATCGCGTCCCAACGCCG
𝑃𝑇𝐸𝑁-UM^b	Forward: TTTTGAGGTGTTTGGGTTTTTGGT	59
	Reverse: ACACAATCACATCCCAACACCA
𝑃𝑇𝐸𝑁-BGS^c	Forward: ATTGGGTATGTTTAGTAGAGTT	60
	Reverse: CAACTCTCAAACTTCCATCATA

a: Methylated primer sequences used for MSP. b: Unmethylated primer sequences used for MSP. c: Primer sequences used for bisulfite genomic sequencing.