An Experimental Approach for the Identification of Conserved Secreted Proteins in Trypanosomatids
Table 1
Primer pairs designed to amplify the genes encoding the 13 T. cruzi putative secreted proteins conserved in trypanosomatids and T. cruzi Tubulin (negative control).
T. cruzi GeneDB Gene ID No.
Primer sequence
F/Rint and F/R product sizes (bp)
MW (kDa
Tc00.1047053506417.30
F CATGAGCTTACTAGTATGTTGTCTCTGGCAGAAGTGTGT
Rint ACGGTGCCCAAAGGCGTGTA
311
R CACACGGAAGCTTTCAATGAT GATGATGATGATGGCGACCAAACCTAGCCATAAG
705
25.7
Tc00.1047053506155.99
F CTGGGGGAATTCATGCGGTGGATTTTTTTGTTACTTGCC
Rint CCGATACGTCCACCACCCCTC
336
R CGTCGGAAGCTTCTAGTGATG GTGGTGGTGATGGACAAGTTCGTGGCATGTAATTG
735
28.1
Tc00.1047053506467.29
F ACACGGACTAGTATGATTGTATTGAATGGAATTTCTGAG
Rint CTAATAGTCCGAAGTCGTTGCG
309
R CTACACAAGCTTTTAGTGATGG TGATGGTGATGGCTGCGCCTCCACACCGTGC
1065
39.7
Tc00.1047053511901.30
F CTGATAGGCACTAGTATGTTTCCGGCGCAGGAATTCCT
Rint CCCCTTTCAGGTGACCATTACAAGAG
316
R GCCGTCAAGCTTTTAGTGGTGG TGGTGGTGATGCTCCGCTCCCAACTTCAAACGA
1041
39.8
Tc00.1047053511871.30
F CTGATAGGCACTAGTATGCGTCGCACTTTATTTTGTCTG
Rint CTCTCCAACTCGTACGGCGA
305
R CTGCAGGCAAGCTTCTAAT GGTGGTGATGGTGATGTTATGATACCGGCATCAAGTCCCC
1269
47.0
Tc00.1047053505789.10
F CGCACTCACTAGTATGCCCTCTGGCAAAGCAACTG
Rint TCACTGCTCCGCCCTGGTTTC
308
R CGCTCCCTCGAGTTAGTGG TGATGGTGGTGATGGGCAGCATTTACCGACCCTGA
1488
53.6
Tc00.1047053509669.70
F GCTCAGCCAAGCTTATGCGCACTTCTTCTGCCGTGT
Rint ATCGGGGAGTTTTGTGCAGGTTGAG
322
R GTGGTCTTCTCGAGTTAGTGAT GGTGGTGGTGATGGTCGACTTTAATGCTCGCGTATA
1944
73.1
Tc00.1047053507765.20
F CTGCCCAGTACTAGTATGTCTGCTAAAGCCTCACGGC
Rint TCCAGGTAGTCACCCATTCCGTG
318
R CTCAGCCAAGCTTTTAATGATG ATGATGATGGTGTCGTCTCGCCTCACAGTGCT
1521
57.2
Tc00.1047053510101.470
F CTGCGCTGGACTAGTATGTCTGTTAAAGCCTCACGGCG
Rint CCATTCCGTGACCGCCGTAGAC
302
R CTCGGTAAGCTTTTAATGATGA TGATGATGGTGTCGTCTCGCCTCACAGTGCT
1518
57.3
Tc00.1047053510443.30
F CTCGCTGGAATTCATGCGGTGGGTGATAGTTGTATTTGC
Rint CGCCAACAACGTAGTTGCCAAG
313
R ACGGACCTCGAGTTAGTGATG GTGGTGGTGATGCTTGTTGAGTTTGGAGCGGCG
612
23.0
Tc00.1047053509799.50
F CGCGGGACTAGTATGAAACAAAAAATGCGACGCAAATTG
Rint GTGAGGATGGGGAACCAAAAGAGTC
297
R CAGCCAAGCTTCTAGTGAT GGTGATGATGATGGACATTCTTCTTCTTTGTAAAGTAG
687
26.5
Tc00.1047053509835.30
F CGCGGCACTAGTATGTATTCATGTTTGTCGCTGAGGC
Rint GCAGCAACGGCAACAAAGAGC
324
R CATGGCAAGCTTTTAGTGA TGGTGGTGGTGATGCTCCTCTCTGGGTTTCCTTCG
2031
71.6
Tc00.1047053509999.10
F CGCGCCACTAGTATGTACGTCGTGCTTTTTTTCGTTT
Rint CGCATATTTCCGCTCCGTTCC
305
R AGCAGTCCAAGCTTTTAGTGAT GGTGATGATGATGGCCGCACCAGCGCTCCAGAA
1227
46.6
Tc00.1047053506563.4
F GGGTGCCACTAGTATGCGTGAGATTGTGTGCGTTCAG
49.6
Rint GGGCGGAAGATCTGCCCGTATG
259
R AGCGCTCAAGCTTTTAGTGATG GTGGTGGTGATGGTACTGCTCCTCCTCGTCGAACT
1329
F: Forward primer including the start codon; Rint: Internal reverse primer for RT-PCR; R: Reverse primer used for the amplification of the full-length ORF. Restriction sites used for cloning in the pTEX vector in italics and the His-Tag sequence in bold. Expected molecular weight of the proteins (deduced from amino acid sequences). Beta-tubulin gene.
