An Experimental Approach for the Identification of Conserved Secreted Proteins in Trypanosomatids

Table 3

Gene ID of L. infantum orthologous genes and primers used for cloning.

Gene ID	Primer sequences(a)	F/R product sizes (bp)	MW(kDa)(b)
LinJ19.0410(c)	F CATGACCACTAGTATGGCCAAAACAGCGCTTCTC	1590	58,4
	R GCAGTCCAAGCTTTTAGTGATGGTGATGATGATGAGGTGTTCTCAGGGGTGACGA
LinJ36.5780(d)	F CATGCTCGACTAGTATGGGGTGCCGCAGTAGCTG
	R GCAGTCCAAGCTTTTAATGATGATGGTGGTGATGATCATCCAACATCTGGCACCGC	738	28

F: Forward primer including the start codon; R: Reverse primer used for the amplification of the full-length ORF.
(a)Restriction sites used for cloning in the pTEX vector in italics and the His-Tag sequence in bold.
(b)Expected molecular weight of the proteins.
(c)Ortholog of LmjF19.0540 and Tc00.1047053505789.10.
(d)Ortholog of LmjF36.5220 and Tc00.1047053506155.99.