Enhanced MALDI-TOF MS Analysis of Phosphopeptides Using an Optimized DHAP/DAHC Matrix
The MALDI mass spectra of the tryptic digest of (a) and (c) -casein and (b) and (d) -casein measured in positive (upper) and negative (lower) ion modes with the DHAP/DAHC matrix. The amount of the tryptic peptides mixture ranged from 5 fmol to 250 fmol on target. The signal intensity is magnified 5-fold in the m/z range from 2400 to 3150 in section a.
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