BioMed Research International / 2010 / Article / Fig 6

Methodology Report

Enhanced MALDI-TOF MS Analysis of Phosphopeptides Using an Optimized DHAP/DAHC Matrix

Figure 6

The MALDI MS/MS spectra of phosphopeptides (a) T 𝛽 1, (b) T 𝛼 3, and (c) T 𝛽 2 (250 fmol for each on target) from 𝛼 -casein and 𝛽 -casein were obtained by MALDI-TOF/TOF MS. The sequential losses of H 3 P O 4 from the parent ions are noted. The fragment patterns of the peptides are indicated in the magnified MS/MS spectra. “X” in the amino sequence shows a dehydroalanine residue converted from a phosphoserine residue by beta-elimination of H 3 P O 4 .

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