Enhanced MALDI-TOF MS Analysis of Phosphopeptides Using an Optimized DHAP/DAHC Matrix
The MALDI MS/MS spectra of phosphopeptides (a) T1, (b) T3, and (c) T2 (250 fmol for each on target) from -casein and -casein were obtained by MALDI-TOF/TOF MS. The sequential losses of from the parent ions are noted. The fragment patterns of the peptides are indicated in the magnified MS/MS spectra. “X” in the amino sequence shows a dehydroalanine residue converted from a phosphoserine residue by beta-elimination of .
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