BioMed Research International / 2010 / Article / Fig 1

Research Article

Reduction of Foxp Cells by Depletion with the PC61 mAb Induces Mortality in Resistant BALB/c Mice Infected with Toxoplasma gondii

Figure 1

Injection of PC61 mAb depletes CD4+CD25+ cells. BALB/c mice (2 animals per group) were injected ip with DPBS (a), 100โ€‰ ๐œ‡ g (b), or 200โ€‰ ๐œ‡ g (c) of purified anti-CD25 mAb (PC61). Two days later, spleen cells were obtained; immunofluorescence was carried out using anti-CD4-FITC and anti-CD25-PE mAbs, and cells were analyzed by FACS. The lymphocyte region was first identified by FSC and SSC characteristics, gated, and 10,000 gated events were captured. Numbers indicate % of CD4+CD25+ cells within the CD4+ population.
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