Reduction of Foxp𝟑⁺ Cells by Depletion with the PC61 mAb Induces Mortality in Resistant BALB/c Mice Infected with Toxoplasma gondii

Figure 3

Depletion with PC61 mAb partially eliminates Foxp3⁺ cells. BALB/c mice were injected with 200 𝜇g of isotype (a) or PC61 (b) mAb ip. Two days later, before infection, animals were bled and immunofluorescence for detection of Foxp3 was carried out. The lymphocyte region was first identified and gated by FSC and SSC characteristics, the CD4⁺ region was subgated, and 10,000 CD4⁺ events were captured. Numbers indicate % of Foxp3⁺ cells within the CD4⁺ population. A representative analysis from one mouse per group is shown.

(a)

(b)