Gene Expression Profiling of Placentas Affected by Pre-Eclampsia

Table 2

Primers and PCR conditions.

Gene target	Forward primer 5^-3^	Reverse primer 5^-3^	TD^a a	TD^a b	TD^a c	TD^a d	PCR^b
			^∘C/c	^∘C/c	^∘C/c	^∘C/c	^∘C/c
Fibulin 1A	aagttggcaggagtggagac	cccccataggtgaatcacag	58/2	57/2	56/2	—	55/35
sFLT-3	taagcacaccacgcccagtc	aagaccgcttgccagctacg	69/2	68/2	67/2	—	66/30
Inhibin	gcttcatgtgggcaaagtcg	ccccctttaagcccacttcc	64/2	62/	60/	59/2	58/30
Leptin	gtccaagctgtgcccatcc	cccaggctgtccaaggtctc	64/2	62/	60/	59/2	58/30

^(a)Touchdown cycle, annealing temperature/number of cycles.
^(b)PCR, annealing temperature/number of cycles.