Functional Differences between the N-Terminal Domains of Mouse and Human Myosin Binding Protein-C
Figure 4
Effects of mouse and human C0, P/A, and C1 domains on thin filament activated myosin S1 ATPase assays at pCa 10 in the absence of myosin S2. 1 M mC0C1 had no effect on ATPase activity ( = 9), whereas 1 M hC0C1 increased ATPase activity ( = 9). The human P/A and C1 domains each activated ATPase activity (mhmC0C1 ( = 7) and mmhC0C1 ( = 5), resp.) to intermediate values. The mouse P/A and C1 domains (hmhC0C1, ( = 5) and hhmC0C1, ( = 6), resp.) depressed ATPase activity when compared to hC0C1. The human C0 domain (hmmC0C1, = 6) had no activating effects on actin-activated S1 ATPase activity, while the mouse C0 domain (mhhC0C1, = 4) did not affect activity when compared to hC0C1. Data are mean ± SD. Asterisks (*) and crosses (†) denote significant differences when compared to mC0C1 and hC0C1, respectively, ().
