BioMed Research International

Research Article

Functional Differences between the N-Terminal Domains of Mouse and Human Myosin Binding Protein-C

Table 2

Primers used to create recombinant chimeric C0C1 proteins.
cMyBP-C domainPrimer pairs (upstream/downstream primer)
Mouse C0 5 -ATCACCATCACCATATGCCGGAGCCAGGGAAGAAACCA - 3 /
5 -AGGGGCCAGCATGGGTTCTGCCTTCTCTGGAGGGGC - 3
Mouse P/A 5 -TCTGAAGTTGCTCCAGGAGCC - 3 /
5 -GTCATCAGGGGCTCCCTGATG - 3
Mouse C1 5 -GGAGCCCCCGATGACCCTATTGGCCTCTTTCTGATGCGA - 3 /
5 -TCAGCTAATTAAGCTTTCATCCAGAACCAATCGCCTCATGGAC - 3
Human C0 5 -ATCACCATCACCATATGCCTGAGCCGGGGAAGAAGC - 3 /
5 -TGGAGCAACTTCAGACTCTGCCTTCTCTGCCTCTATGAC - 3
Human P/A 5 -CCCATGCTGGCCCCTGC - 3 /
5 -GTCATCGGGGGCTCCAGG - 3
Human C1 5 -GGAGCCCCTGATGACCCCATTGGCCTCTTCGTGATGC - 3 /
5 -TCAGCTAATTAAGCTTTCATCCGGTGCCCATGGCCT - 3