Regulation of Adrenal Aldosterone Production by Serine Protease Prostasin

Figure 1

(a) Effect of prostasin on human CYP11B2 promoter activity in H295R cells. Cells were transfected with human CYP11B2 promoter-luciferase construct (pB2-1521) for 24 hours and then treated with vehicle or prostasin for another 24 hours. (b) Dose-dependent effect of prostasin on CYP11B2 mRNA expression in H295R cells. Cells were treated with increasing doses of prostasin for 24 hours. (c) Time course of CYP11B2 mRNA induction by prostasin in H295R cells. Cells were treated with 100 μg/mL of prostasin for 0, 3, 6, and 24 hours. (d) Dose-dependent effect of prostasin on aldosterone production in H295R cells. Cells were treated with increasing concentrations of prostasin for 48 hours. Values are means ± SD (𝑛=6). ^∗𝑃<.05, ^†𝑃<.01, and ^#𝑃<.001.

(a)

(b)

(c)

(d)